Ukusetyenziswa kwetekhnoloji yokupholisa i-thermoelectric kwizixhobo ze-PCR

Ukusetyenziswa kwetekhnoloji yokupholisa i-thermoelectric kwizixhobo ze-PCR

Ukusetyenziswa kwetekhnoloji yokupholisa i-thermoelectric kwizixhobo ze-PCR ikakhulu kukulawula ubushushu. Inzuzo yayo ephambili kukulawula ubushushu ngokukhawuleza nangokuchanekileyo, okuqinisekisa impumelelo yezilingo zokukhulisa i-DNA.

Iimeko eziphambili zesicelo

1. Ulawulo lobushushu oluchanekileyo

Isixhobo se-PCR kufuneka sijikeleze izigaba ezintathu: ukuguquguquka kobushushu obuphezulu (90-95℃), ukufakelwa kobushushu obuphantsi (55-65℃), kunye nokwandiswa kobushushu obufanelekileyo (70-75℃). Iindlela zemveli zokufriji kunzima ukuhlangabezana nemfuneko yokuchaneka kwe-±0.1℃. Itekhnoloji yokupholisa i-peltier yokupholisa i-thermoelectric ifezekisa ulawulo lobushushu obuphezulu be-millisecond ngesiphumo sePeltier, ithintela ukungaphumeleli kokukhulisa okubangelwa ngumahluko wobushushu be-2℃.

2. Ukupholisa nokufudumeza ngokukhawuleza

Iimodyuli zokupholisa ze-thermoelectric, iimodyuli ze-thermoelectric, izixhobo ze-peltier, iimodyuli ze-peltier zinokufikelela kwisantya sokupholisa se-3 ukuya kwi-5 degrees Celsius ngomzuzwana, nto leyo enciphisa kakhulu umjikelo wovavanyo xa kuthelekiswa ne-2 degrees Celsius ngomzuzwana yee-compressors zemveli. Umzekelo, isixhobo se-96-well PCR sisebenzisa itekhnoloji yokulawula ubushushu bezoni ukuqinisekisa ubushushu obuhambelanayo kuzo zonke iindawo zemithombo kunye nokuphepha umahluko wobushushu be-2℃ obangelwa ziziphumo zomphetho.

3. Ukuphucula ukuthembeka kwezixhobo

Iimodyuli zokupholisa ze-thermoelectric, iimodyuli ze-peltier, iimodyuli ze-peltier, iimodyuli ze-TEC zeBeijing Huimao Cooling Equipment Co., Ltd. ziye zaba zizixhobo zokulawula ubushushu eziphambili zezixhobo ze-PCR ngenxa yokuthembeka kwazo okuphezulu. Ubungakanani bayo obuncinci kunye neempawu zayo ezingenangxolo zenza ukuba ifaneleke kwiimfuno ezichanekileyo zezixhobo zonyango.

Amatyala aqhelekileyo okusetyenziswa

Isixhobo sokufumanisa ubungakanani be-PCR esine-fluorescence engama-96: Sidityaniswe nemodyuli yokupholisa ye-thermoelectric, imodyuli ye-TEC, isixhobo se-peltier, iimodyuli ze-peltier, senza kube lula ukulawula ubushushu obuchanekileyo beesampuli ze-high-throughput kwaye sisetyenziswa kakhulu kwiindawo ezifana nohlalutyo lokubonakaliswa kwezakhi zofuzo kunye nokufunyanwa kwezifo.

Iifriji zonyango eziphathekayo: ukupholisa ngobushushu obuphezulu, ukupholisa nge-peltier iifriji zonyango eziphathekayo ezisetyenziselwa ukugcina iimveliso ezifana neegonyo kunye namayeza afuna indawo enobushushu obuphantsi, ukuqinisekisa uzinzo lobushushu ngexesha lokuthuthwa.

Izixhobo zonyango lwelaser:

Iimodyuli zokupholisa ze-thermoelectric, izinto ze-peltier, iimodyuli ze-thermoelectric zipholisa i-laser emitter ukunciphisa umngcipheko wokutsha kolusu kunye nokuphucula ukhuseleko lonyango.

Iinkcukacha ze-TEC1-39109T200

Ubushushu obushushu ecaleni bungama-30 C,

Ubungakanani: 9A

Umax: 46V

Ubuninzi beQ: 246.3W

I-ACR: 4±0.1Ω（Ta= 23 C）

Ubuninzi beDelta T: 67 -69C

Ubungakanani: 55x55x3.5-3.6mm

Iinkcukacha zeTES1-15809T200

Ubushushu obushushu ecaleni: 30 C,

Ubungakanani: 9.2A,

Ubuninzi: 18.6V

Ubuninzi: 99.5 W

Ubuninzi beDelta T: 67 C

I-ACR：1.7 ±15% Ω （1.53 ukuya kwi-1.87 Ohm）

Ubungakanani: 77 × 16.8 × 2.8mm

Ucingo: Ucingo lwe-silicone lwe-18 AWG okanye olulinganayo olufakwe i-Sn-plated phezu komphezulu, ubushushu obuphezulu obuxhathisayo yi-200℃