Ukusetyenziswa kweteknoloji yokupholisa i-thermoelectric kwizixhobo ze-PCR

Ukusetyenziswa kweteknoloji yokupholisa i-thermoelectric kwizixhobo ze-PCR

Ukusetyenziswa kweteknoloji yokupholisa i-thermoelectric kwizixhobo ze-PCR ikakhulu kulawulo lobushushu. Inzuzo yayo engundoqo kukukwazi ukulawula ubushushu obukhawulezayo nobuchanekileyo, obuqinisekisa izinga lempumelelo yovavanyo lokukhulisa i-DNA.

Iimeko zesicelo eziphambili

1. Ulawulo oluchanekileyo lobushushu

Isixhobo se-PCR sidinga ukujikeleza ngezigaba ezintathu: i-denaturation yobushushu obuphezulu (90-95 ℃), i-annealing yobushushu obuphantsi (55-65℃), kunye nokwandiswa kweqondo lokushisa eliphezulu (70-75℃). Iindlela zesiqhelo zokukhenkcisa kunzima ukuhlangabezana neemfuno ezichanekileyo ze-±0.1℃. Ukupholisa kwe-Thermoelectric, itekhnoloji yokupholisa i-peltier ifezekisa ulawulo lobushushu benqanaba le-millisecond ngesiphumo sePeltier, ukuthintela ukusilela kokukhulisa okubangelwa ngumahluko we-2 ℃.

2. Ukupholisa ngokukhawuleza kunye nokufudumeza

Iimodyuli zokupholisa i-thermoelectric, iimodyuli ze-thermoelectric, izixhobo ze-peltier, iimodyuli ze-peltier zinokufikelela izinga lokupholisa kwi-3 ukuya kwi-5 degrees Celsius ngomzuzwana, ukunciphisa kakhulu umjikelezo wokulinga xa kuthelekiswa ne-2 degrees Celsius ngesekhondi ye-compressors yendabuko. Umzekelo, isixhobo se-PCR esine-96-well sisebenzisa itekhnoloji yokulawula ubushushu bendawo ukuze kuqinisekiswe amaqondo obushushu angaguqukiyo kuzo zonke iindawo zomthombo kwaye unqande umahluko wobushushu obuyi-2℃ obangelwa ziziphumo zomda.

3. Ukuphucula ukuthembeka kwezixhobo

Iimodyuli zokupholisa i-thermoelectric, iimodyuli ze-peltier, i-peltier lements, iimodyuli ze-TEC ze-Beijing Huimao Cooling Equipment Co., Ltd. ziye zaba ngamacandelo angundoqo okulawula ubushushu bezixhobo ze-PCR ngenxa yokuthembeka kwazo okuphezulu. Ubungakanani bayo obuncinci kunye neempawu ezingenangxolo ziyenza ilungele iimfuno ezichanekileyo zezixhobo zonyango.

Iimeko zesicelo eziqhelekileyo

I-96-well fluorescence quantitative detector ye-PCR: Idityaniswe nemodyuli yokupholisa i-thermoelectric, imodyuli ye-TEC, isixhobo se-peltier, iimodyuli ze-peltier ivumela ulawulo oluchanekileyo lobushushu beesampuli eziphezulu ze-high-throughput kwaye isetyenziswa ngokubanzi kwiinkalo ezifana nohlalutyo lwe-gene expression kunye nokufumanisa i-pathogen.

Izikhenkcezisi zonyango eziphathwayo: ukupholisa umbane oshushu, ukupholisa kwe-peltier iifriji zonyango eziphathwayo ezisetyenziselwa ukugcina iimveliso ezinje ngogonyo kunye namayeza afuna imekobume yobushushu obuphantsi, eqinisekisa uzinzo lobushushu ngexesha lothutho.

Izixhobo zonyango lweLaser:

Iimodyuli zokupholisa ze-Thermoelectric, izinto ze-peltier, iimodyuli ze-thermoelectric zipholisa i-laser emitter ukunciphisa umngcipheko wokutsha kwesikhumba kunye nokuphucula ukhuseleko lonyango.

Inkcazo ye-TEC1-39109T200

Ubushushu becala elishushu yi-30 C,

Ubukhulu: 9A

Umax: 46V

Ubukhulu: 246.3W

I-ACR: 4±0.1Ω (Ta= 23 C)

Delta T max: 67 -69C

Ubungakanani: 55x55x3.5-3.6mm

Inkcazo ye-TES1-15809T200

Ubushushu becala elishushu: 30 C,

Ubukhulu: 9.2A

Umax: 18.6V

Ubukhulu: 99.5 W

Ubuninzi beDelta T: 67C

I-ACR:1.7 ±15% Ω (1.53 ukuya ku-1.87 Ohm)

Ubungakanani: 77×16.8×2.8mm

Ucingo: I-18 AWG i-silicone ucingo okanye i-Sn-plated elinganayo phezu komhlaba, i-Resistance yokushisa ephezulu 200 ℃