Ukupholisa iPeltier (iteknoloji yokupholisa i-thermoelectric esekelwe kwisiphumo sePeltier) ibe yenye yeendlela eziphambili zenkqubo yokulawula ubushushu kwizixhobo ze-PCR (polymerase chain reaction) ngenxa yempendulo yayo ekhawulezayo, ulawulo lobushushu oluchanekileyo, kunye nobukhulu obuncinci, obuchaphazela kakhulu ukusebenza kakuhle, ukuchaneka, kunye neemeko zokusetyenziswa kwe-PCR. Oku kulandelayo luhlalutyo oluneenkcukacha lwezicelo ezithile kunye neenzuzo zokupholisa i-thermoelectric (ukupholisa i-peltier) ukusuka kwiimfuno eziphambili ze-PCR:
I. Iimfuno eziphambili zoLawulo loBushushu kwiTekhnoloji yePCR
Inkqubo ephambili ye-PCR ngumjikelo ophindaphindayo we-denaturation (90-95℃), i-annealing (50-60℃), kunye nolwandiso (72℃), oluneemfuneko ezingqongqo kakhulu kwinkqubo yolawulo lobushushu.
Ukunyuka nokwehla kobushushu ngokukhawuleza: Nciphisa ixesha lomjikelo omnye (umzekelo, kuthatha imizuzwana embalwa kuphela ukwehla ukusuka kwi-95℃ ukuya kwi-55℃), kwaye uphucule ukusebenza kakuhle kokuphendula;
Ulawulo lobushushu oluchanekileyo: Ukuphambuka kwe-±0.5℃ kubushushu bokutsalela amanzi kunokubangela ukwandiswa okungacwangciswanga, kwaye kufuneka kulawulwe ngaphakathi kwe-±0.1℃.
Ukufana kobushushu: Xa iisampuli ezininzi zisabela ngaxeshanye, umahluko wobushushu phakathi kweesampuli zeWells kufuneka ube yi-≤0.5℃ ukuthintela ukuphambuka kweziphumo.
Uhlengahlengiso lweMiniaturization: I-PCR ephathekayo (njengeemeko zePOCT zovavanyo kwindawo) kufuneka ibe ncinci ngobukhulu kwaye ingabi nazinto zokuguguleka koomatshini.
II. Usetyenziso oluphambili lokupholisa i-thermoelectric kwi-PCR
I-thermoelectric Cooler TEC, i-Thermoelectric cooling module, i-peltier module ifezekisa "ukutshintsha kabini kokufudumeza nokupholisa" ngokusebenzisa i-direct current, ihambelana ngokugqibeleleyo neemfuno zolawulo lobushushu ze-PCR. Ukusetyenziswa kwayo okukhethekileyo kubonakala kwezi zinto zilandelayo:
1. Ukunyuka nokuwa kobushushu ngokukhawuleza: Nciphisa ixesha lokuphendula
Umgaqo: Ngokutshintsha indlela yombane, imodyuli ye-TEC, imodyuli ye-thermoelectric, isixhobo se-peltier sinokutshintsha ngokukhawuleza phakathi kwendlela "yokufudumeza" (xa umbane usiya phambili, isiphelo esifunxa ubushushu semodyuli ye-TEC, imodyuli ye-peltier iba sisiphelo esikhupha ubushushu) kwaye "ipholisa" (xa umbane ubuyela umva, isiphelo esikhupha ubushushu siba sisiphelo esifunxa ubushushu), ixesha lokuphendula lidla ngokuba ngaphantsi kwesekhondi enye.
Iingenelo: Iindlela zemveli zokufrijisa (ezifana neefeni kunye neecompressor) zixhomekeke ekuqhubeni ubushushu okanye ekushukumeni koomatshini, kwaye amazinga okufudumeza nokupholisa adla ngokuba ngaphantsi kwe-2℃/s. Xa i-TEC idityaniswe neebhloko zesinyithi eziqhuba ubushushu obuphezulu (ezifana ne-copper kunye ne-aluminium alloy), inokufikelela kwinqanaba lokufudumeza nokupholisa le-5-10℃/s, inciphise ixesha lomjikelo we-PCR enye ukusuka kwimizuzu engama-30 ukuya ngaphantsi kwemizuzu eli-10 (njengakwizixhobo ze-PCR ezikhawulezayo).
2. Ulawulo lobushushu oluchanekileyo: Ukuqinisekisa ukucaciswa kokukhulisa
Umgaqo: Amandla okukhupha (ubushushu/ubushushu) kwimodyuli ye-TEC, imodyuli yokupholisa ye-thermoelectric, imodyuli ye-thermoelectric ihambelana ngokuthe ngqo nobushushu bangoku. Idityaniswe nee-sensors zobushushu ezichanekileyo (ezifana nokumelana neplatinum, i-thermocouple) kunye nenkqubo yolawulo lwempendulo ye-PID, umbane ungalungiswa ngexesha langempela ukuze kufezekiswe ulawulo lobushushu oluchanekileyo.
Iingenelo: Ukuchaneka kokulawula ubushushu kunokufikelela kwi-±0.1℃, okungaphezulu kakhulu kunoko kumanzi okuhlamba okanye kwifriji yecompressor yesiqhelo (±0.5℃). Umzekelo, ukuba ubushushu obujoliswe kubo ngexesha lesigaba sokutsalwa kwe-annealing yi-58℃, imodyuli ye-TEC, imodyuli ye-thermoelectric, i-peltier cooler, i-peltier element ingagcina obu bushushu ngokuzinzileyo, iphephe ukubopha okungangqalanga kwee-primers ngenxa yokuguquguquka kobushushu kunye nokuphucula kakhulu ukucaciswa kwe-amplification.
3. Uyilo oluncinci: Ukukhuthaza uphuhliso lwe-PCR ephathekayo
Umgaqo: Umthamo wemodyuli ye-TEC, i-peltier element, isixhobo se-peltier ziisentimitha ezimbalwa kuphela (umzekelo, imodyuli ye-TEC ye-10×10mm, imodyuli yokupholisa ye-thermoelectric, imodyuli ye-peltier inokuhlangabezana neemfuno zesampuli enye), ayinazo iindawo ezihamba ngoomatshini (ezifana nepiston yecompressor okanye ii-fan blades), kwaye ayifuni friji.
Iingenelo: Xa izixhobo zePCR zemveli zixhomekeke kwi-compressors ukuze zipholise, umthamo wazo uhlala ungaphezulu kwe-50L. Nangona kunjalo, izixhobo zePCR eziphathekayo ezisebenzisa i-thermoelectric cooling module, i-thermoelectric module, i-peltier module, i-TEC module zinokuncitshiswa zibe ngaphantsi kwe-5L (njengezixhobo eziphathwa ngesandla), nto leyo ezenza zifaneleke kuvavanyo lwasentsimini (njengokuhlolwa kwindawo ngexesha lobhubhane), uvavanyo lweklinikhi ecaleni kwebhedi, kunye nezinye iimeko.
4. Ukufana kobushushu: Qinisekisa ukuhambelana phakathi kweesampuli ezahlukeneyo
Umgaqo: Ngokulungiselela iiseti ezininzi zee-TEC arrays (ezifana nee-96 micro TECs ezihambelana nepleyiti ye-96-well), okanye xa zidityaniswe neebhloko zesinyithi ezabelana ngobushushu (izinto eziqhuba ubushushu obuphezulu), ukuphambuka kobushushu okubangelwa kukwahluka komntu ngamnye kwii-TECs kunokulungiswa.
Iingenelo: Umahluko wobushushu phakathi kwe-sample Wells unokulawulwa ngaphakathi kwe-±0.3℃, kuthintelwe umahluko wokusebenza kakuhle kokukhulisa okubangelwa kubushushu obungaguquguqukiyo phakathi kwe-edge Wells kunye ne-central Wells, kwaye kuqinisekiswe ukuthelekiswa kweziphumo zesampulu (njengokuhambelana kwamaxabiso e-CT kwi-real-time fluorescence quantitative PCR).
5. Ukuthembeka nokugcinwa kwayo: Nciphisa iindleko zexesha elide
Umgaqo: I-TEC ayinazo izinto ezigugileyo, inobomi obungaphezulu kweeyure ezili-100,000, kwaye ayifuni ukutshintshwa rhoqo kweefriji (ezifana neFreon kwiicompressor).
Iingenelo: Ixesha eliqhelekileyo lobomi besixhobo se-PCR esipholileyo yi-compressor yendabuko limalunga neminyaka emi-5 ukuya kweyesi-8, ngelixa inkqubo ye-TEC inokwandisa ukuya kwiminyaka engaphezu kwe-10. Ngaphezu koko, ukugcinwa kufuna kuphela ukucoca isinki yobushushu, okunciphisa kakhulu iindleko zokusebenza kunye nokugcinwa kwezixhobo.
III. Imingeni kunye nokuphuculwa kwezicelo
Ukupholisa i-semiconductor akugqibelelanga kwi-PCR kwaye kufuna ukulungiswa okujoliswe kuko:
Ingxaki yokusasaza ubushushu: Xa i-TEC iphola, ubushushu obuninzi buyaqokelelana ekupheleni kokukhululwa kobushushu (umzekelo, xa ubushushu buhla ukusuka kwi-95℃ ukuya kwi-55℃, umahluko wobushushu ufikelela kwi-40℃, kwaye amandla okukhupha ubushushu ayanda kakhulu). Kubalulekile ukuyidibanisa nenkqubo yokusasaza ubushushu esebenzayo (njengezitshingi zobushushu zekopolo + iifeni zeturbine, okanye iimodyuli zokupholisa ulwelo), kungenjalo kuya kukhokelela ekunciphiseni ukusebenza kakuhle kokupholisa (kwaye nomonakalo ogqithisileyo).
Ulawulo lokusetyenziswa kwamandla: Phantsi komahluko omkhulu wobushushu, ukusetyenziswa kwamandla e-TEC kuphezulu kakhulu (umzekelo, amandla e-TEC esixhobo se-PCR esine-96-well angafikelela kwi-100-200W), kwaye kuyimfuneko ukunciphisa ukusetyenziswa kwamandla okungasebenzi kakuhle ngokusebenzisa ii-algorithms ezikrelekrele (ezifana nolawulo lobushushu oluqikelelayo).
Iv. Amatyala okusetyenziswa okusebenzayo
Okwangoku, izixhobo zePCR eziphambili (ingakumbi izixhobo zePCR ezibonisa ubungakanani be-fluorescence) ngokubanzi zisebenzise itekhnoloji yokupholisa ye-semiconductor, umzekelo:
Izixhobo ezikumgangatho welebhu: Isixhobo se-PCR esinomlinganiselo we-96-well fluorescence quantitative se-brand ethile, esine-TEC temperature control, esinezinga lokufudumeza nokupholisa elifikelela kwi-6℃/s, ukuchaneka kokulawula ubushushu be-±0.05℃, kunye nokuxhasa ukufunyanwa kwe-384-well high-throughput.
Isixhobo esiphathwayo: Isixhobo esithile se-PCR esiphathwayo (esinobunzima obungaphantsi kwe-1kg), esisekelwe kuyilo lwe-TEC, sinokugqiba ukubhaqwa kwe-coronavirus entsha kwimizuzu engama-30 kwaye sifanelekile kwiimeko ezikwindawo ezifana nezikhululo zeenqwelo-moya kunye noluntu.
Isishwankathelo
Ukupholisa nge-thermoelectric, kunye neenzuzo ezintathu eziphambili zokusabela ngokukhawuleza, ukuchaneka okuphezulu kunye nokunciphisa i-aturization, kusombulule iindawo eziphambili zetekhnoloji ye-PCR ngokubhekiselele ekusebenzeni kakuhle, ukucacisa kunye nokuguquguquka kwendawo, ibe yiteknoloji eqhelekileyo yezixhobo zanamhlanje ze-PCR (ingakumbi izixhobo ezikhawulezayo neziphathwayo), kunye nokukhuthaza i-PCR ukusuka kwilebhu ukuya kwiindawo ezibanzi zokusetyenziswa ezifana nebhedi yeklinikhi kunye nokufunyanwa kwindawo.
I-TES1-15809T200 yomatshini we-PCR
Ubushushu obushushu ecaleni: 30 C,
Ubungakanani: 9.2A,
Ubuninzi: 18.6V
Ubuninzi: 99.5 W
Ubuninzi beDelta T: 67 C
I-ACR：1.7 ±15% Ω （1.53 ukuya kwi-1.87 Ohm）
Ubungakanani: 77 × 16.8 × 2.8mm
Ixesha lokuthumela: Agasti-13-2025
