Ukupholisa kwePeltier (iteknoloji yokupholisa i-thermoelectric esekelwe kwimpembelelo yePeltier) iye yaba yenye yezobuchwepheshe obuphambili benkqubo yokulawula ubushushu kwi-PCR (i-polymerase chain reaction) izixhobo ngenxa yokusabela kwayo ngokukhawuleza, ulawulo oluchanekileyo lobushushu, kunye nobukhulu obudibeneyo, obuchaphazela kakhulu ukusebenza kakuhle, ukuchaneka, kunye neemeko zesicelo se-PCR. Oku kulandelayo lucazululo oluneenkcukacha lwezicelo ezithile kunye noncedo lokupholisa thermoelectric(i-peltier cooling) eqala kwiimfuno eziphambili zePCR:
I. IiMfuno ezingundoqo zoLawulo loBuqondo kwi-PCR Technology
Inkqubo engundoqo ye-PCR ngumjikelo ophindaphindiweyo we-denaturation (90-95℃), i-annealing (50-60℃), kunye nokwandiswa (72℃), eneemfuno ezingqongqo kakhulu zenkqubo yokulawula ubushushu.
Ukunyuka nokuhla kweqondo lobushushu ngokukhawuleza: Ukunciphisa ixesha lomjikelo omnye (umzekelo, kuthatha imizuzwana embalwa kuphela ukuhla ukusuka ku-95℃ ukuya kuma-55℃), kunye nokuphucula ukusebenza kakuhle kokusabela;
Ulawulo oluchanekileyo lobushushu obuphezulu: Ukutenxa kwi-±0.5℃ kwiqondo lobushushu le-annealing kunokukhokelela kulwandiso olungangqalanga, kwaye kufuneka ilawulwe ngaphakathi ±0.1℃.
Ubushushu obufanayo: Xa iisampulu ezininzi zisabela ngaxeshanye, umahluko weqondo lobushushu phakathi kwesampulu ye Wells kufuneka ube ≤0.5℃ ukunqanda ukutenxa kweziphumo.
Ukulungiswa kweMiniaturization: I-PCR ephathekayo (efana novavanyo lwe-POCT kwisiza iimeko) kufuneka ilingane ngobukhulu kwaye ingabi namalungu okunxiba oomatshini.
II. Usetyenziso olungundoqo lokupholisa i-thermoelectric kwi-PCR
I-Thermoelectric Cooler TEC, imodyuli yokupholisa i-Thermoelectric, imodyuli ye-peltier ifezekisa "ukutshintshwa kwe-bidirectional yokufudumeza kunye nokupholisa" ngokuhambelana ngqo, ngokuhambelana ngokugqibeleleyo neemfuno zokulawula ubushushu be-PCR. Ukusetyenziswa kwayo okuthe ngqo kubonakaliswa kule miba ilandelayo:
1. Ukunyuka nokwehla kweqondo lobushushu ngokukhawuleza: Nciphisa ixesha lokusabela
Umgaqo: Ngokutshintsha isalathiso semodyuli yangoku, imodyuli ye-TEC, imodyuli ye-thermoelectric, isixhobo se-peltier sinokutshintsha ngokukhawuleza phakathi "kokufudumala" (xa i-current ibheka phambili, isiphelo esibamba ubushushu semodyuli ye-TEC, imodyuli ye-peltier iba sisiphelo sokukhupha ukushisa) kunye "nokupholisa" (xa okwangoku ijika, i-modebs ekhupha ukushisa ngokuqhelekileyo iba yi-modebs yokuphelisa ukushisa, kunye ne-modebs yokuphelisa ukushisa ngokuqhelekileyo iba yi-modebs. 1 umzuzwana.
Izinto eziluncedo: Iindlela zesiqhelo zokukhenkcisa (ezifana neefeni kunye nezicinezeli) zixhomekeke ekuqhubeni ubushushu okanye kwintshukumo yomatshini, kwaye amazinga okufudumeza kunye nokupholisa akholisa ukuba ngaphantsi kwe-2℃/s. Xa i-TEC idityaniswe kunye neebhloko zetsimbi ze-thermal conductivity eziphezulu (ezifana nobhedu kunye ne-aluminium alloy), inokufikelela kwinqanaba lokufudumeza kunye nokupholisa kwe-5-10 ℃ / s, ukunciphisa ixesha lomjikelo we-PCR enye ukusuka kwimizuzu engama-30 ukuya ngaphantsi kwemizuzu eyi-10 (njengezixhobo zePCR ezikhawulezayo).
2. Ukulawulwa kobushushu obuchanekileyo: Ukuqinisekisa ukucaciswa kokukhulisa
Umgaqo: Amandla okuphuma (ukufudumeza / ukupholisa ngamandla) kwimodyuli ye-TEC, imodyuli yokupholisa i-thermoelectric, imodyuli ye-thermoelectric ihambelana ngokuhambelana nobukhulu bangoku. Idibaniswe ne-high-precision sensors yeqondo lokushisa (ezifana nokumelana ne-platinum, i-thermocouple) kunye nenkqubo yokulawula impendulo ye-PID, okwangoku kunokulungiswa ngexesha langempela ukufezekisa ulawulo oluchanekileyo lokushisa.
Izinto eziluncedo: Ukuchaneka kokulawula ubushushu kunokufikelela kwi-±0.1℃, engaphezulu kakhulu kunokuhlamba ulwelo lwesintu okanye isibandisi secompressor (±0.5℃). Umzekelo, ukuba iqondo lobushushu ekujoliswe kulo ngexesha lenqanaba le-annealing yi-58 ℃, imodyuli ye-TEC, imodyuli yombane we-thermoelectric, i-peltier cooler, i-peltier element inokugcina eli qondo lobushushu ngokuzinza, inqanda ukubophelela okungangqalanga kwe-primers ngenxa yokuguquguquka kobushushu kunye nokuphucula ngokuphawulekayo ukucaciswa kokukhulisa.
3. Uyilo oluncinci: Ukukhuthaza uphuhliso lwePCR ephathekayo
Umgaqo: Umthamo wemodyuli ye-TEC, i-peltier element, isixhobo se-peltier simbalwa seesentimitha zesikwere (umzekelo, imodyuli ye-TEC ye-10 × 10mm, imodyuli yokupholisa i-thermoelectric, imodyuli ye-peltier inokuhlangabezana neemfuno zesampulu enye), ayinayo i-mechanical moves (njenge-piston ye-compressor okanye i-fan blades), kwaye ayifuni i-refrigerant.
Izinto eziluncedo: Xa izixhobo zePCR zemveli zixhomekeke kwiicompressors zokupholisa, umthamo wazo udla ngokuba ngaphezulu kwe-50L. Nangona kunjalo, izixhobo ze-PCR eziphathwayo ezisebenzisa imodyuli yokupholisa i-thermoelectric, imodyuli ye-thermoelectric, imodyuli ye-peltier, imodyuli ye-TEC ingancitshiswa ibe ngaphantsi kwe-5L (njengezixhobo eziphathwa ngesandla), okwenza ukuba zilungele ukuvavanya intsimi (njengokuhlolwa kwendawo ngexesha le-epidemics), ukuvavanywa kwebhedi yekliniki, kunye nezinye iimeko.
4. Ubushushu obufanayo: Qinisekisa ukuhambelana phakathi kweesampuli ezahlukeneyo
Umgaqo: Ngokucwangcisa iiseti ezininzi ze-TEC (ezifana ne-96 micro TECs ehambelana ne-96-well plate), okanye ngokudibanisa neebhloko zetsimbi zokwabelana ngobushushu (izixhobo eziphezulu ze-thermal conductivity), ukuphambuka kweqondo lokushisa okubangelwa ukungafani komntu ngamnye kwi-TECs kunokulungiswa.
Izinto eziluncedo: Umahluko wobushushu phakathi kwesampulu ye Wells inokulawulwa ngaphakathi ± 0.3 ℃, ukuthintela ukuchaneka kobuchule bokukhulisa ukukhulisa okubangelwa ngamaqondo obushushu angahambelaniyo phakathi komphetho weeWell kunye nombindi we Wells, kunye nokuqinisekisa ukuthelekiswa kweziphumo zesampulu (ezifana nokuhambelana kwamaxabiso eCT kwi-real-time fluorescence quantitative PCR).
5. Ukuthembeka kunye nokugcinwa: Ukunciphisa iindleko zexesha elide
Umgaqo: I-TEC ayinamalungu okunxiba, inobomi obungaphezu kweeyure ze-100,000, kwaye ayifuni ukutshintshwa rhoqo kweefriji (ezifana neFreon kwi-compressors).
Izinto eziluncedo: Umndilili wobomi besixhobo se-PCR esipholiswe sisicinezeli esiqhelekileyo simalunga neminyaka emi-5 ukuya kwe-8, ngelixa inkqubo ye-TEC inokwandisa ukuya kuthi ga kwiminyaka eli-10. Ngaphezu koko, ukugcinwa kufuna kuphela ukucoca i-heat sink, ukunciphisa kakhulu iindleko zokusebenza kunye nokugcinwa kwezixhobo.
III. Imiceli mngeni kunye noLungiso kwizicelo
Ukupholisa kweSemiconductor akugqibelelanga kwi-PCR kwaye kufuna ukuphuculwa okujoliswe kuko:
I-bottleneck yokutshatyalaliswa kobushushu: Xa i-TEC ipholisa, ubuninzi bobushushu buqokelela ekupheleni kokukhululwa kobushushu (umzekelo, xa ubushushu behla ukusuka kwi-95 ℃ ukuya kwi-55 ℃, umahluko weqondo lokushisa ufikelela kwi-40 ℃, kwaye amandla okukhululwa kobushushu akhula kakhulu). Kuyimfuneko ukuyidibanisa kunye nenkqubo esebenzayo yokutshatyalaliswa kobushushu (ezifana ne-copper heat sinks + fans turbine, okanye iimodyuli zokupholisa ulwelo), ngaphandle koko kuya kukhokelela ekunciphiseni kokupholisa kakuhle (kunye nomonakalo ogqithisayo).
Ukulawulwa kokusetyenziswa kwamandla: Ngaphantsi komlinganiselo omkhulu weqondo lokushisa, ukusetyenziswa kwamandla kwe-TEC kuphezulu kakhulu (umzekelo, amandla e-TEC esixhobo se-PCR ye-96-well anokufikelela kwi-100-200W), kwaye kuyimfuneko ukunciphisa ukusetyenziswa kwamandla okungenakusebenza ngokusebenzisa i-algorithms ehlakaniphile (njengokulawula ubushushu obuqikelelweyo).
Iv. Iimeko zeSicelo esiSebenzayo
Okwangoku, izixhobo zePCR eziqhelekileyo (ingakumbi izixhobo zePCR zexesha lokwenyani le-fluorescence) ziye zamkela itekhnoloji yokupholisa ye-semiconductor, umzekelo:
Izixhobo zodidi lweLebhu: Isixhobo sePCR esine-96-well fluorescence quantitative yohlobo oluthile, esinolawulo lweqondo lobushushu lwe-TEC, nezinga lokufudumeza nokupholisa ukuya kuthi ga kwi-6℃/s, ulawulo lobushushu oluchanekileyo oluyi-±0.05℃, kunye nokuxhasa ukufunyanwa kwe-384-well-high-throughput.
Isixhobo esiphathwayo: Isixhobo esithile sePCR esiphathwa ngesandla (esinobunzima obungaphantsi kwe-1kg), ngokusekwe kuyilo lwe-TEC, sinokugqiba ukubhaqwa kwe-coronavirus yenoveli kwimizuzu engama-30 kwaye silungele iimeko ezikwisiza ezifana nezikhululo zeenqwelo moya kunye noluntu.
Isishwankathelo
Ukupholisa kwe-Thermoelectric, kunye neenzuzo zayo ezintathu ezingundoqo zokusabela ngokukhawuleza, ukuchaneka okuphezulu kunye ne-miniaturization, isombulule iintlungu eziphambili zeteknoloji ye-PCR ngokusebenza kakuhle, ukuchaneka kunye nokuguquguquka kwendawo, ibe yitekhnoloji esemgangathweni yezixhobo zanamhlanje ze-PCR (ingakumbi izixhobo ezikhawulezayo neziphathwayo), kunye nokukhuthaza i-PCR ukusuka kwibhubhoratri ukuya kwiinkalo zezicelo ezibanzi ezifana nokufunyanwa kwebhedi kunye ne-bed-site.
TES1-15809T200 kumatshini PCR
Ubushushu becala elishushu: 30 C,
Ubukhulu: 9.2A
Umax: 18.6V
Ubukhulu: 99.5 W
Ubuninzi beDelta T: 67C
I-ACR:1.7 ±15% Ω (1.53 ukuya ku-1.87 Ohm)
Ubungakanani: 77×16.8×2.8mm
Ixesha lokuposa: Aug-13-2025
